The Peresopnytsia Gospels (Ukrainian: Пересопницьке Євангеліє, Peresopnytske Yevanheliie), dating from the 16th century, is one of the most intricate surviving East Slavic manuscripts. It was made between 15 August 1556 and 29 August 1561, at the Monastery of the Holy Trinity in Iziaslav, and the Monastery of the Mother of God in , Volyn'. The scribe was , son of an archpriest from , who worked under the direction of , the archimandrite of the Peresopnytsia Monastery.

The manuscript is a Gospel Book containing only the four Gospels of the New Testament, and is ornamented with Glagolitic characters, which were influenced by the Italian Renaissance style. This is the first known example of a vernacular Old Ukrainian translation of the canonical text of the Scriptures.